prostate cancer tissue microarray (pr1921a)  (Xi'an Tianlong Science)

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Targeting the SPOCK1-snail/slug axis-mediated epithelial-to-mesenchymal transition by apigenin contributes to repression of prostate cancer metastasis

doi: 10.1186/s13046-019-1247-3

Figure Lengend Snippet: Interplay between SPOCK1 and epithelial-to-mesenchymal transition (EMT) marker expressions is involved in apigenin (API)-mediated inhibition of cell motility. a Western blot analysis of the protein level of SPOCK1 in PNT2 prostate epithelial cells and LNCaP, DU145, PC-3, and PC-3 M prostate cancer cell lines. The protein level of SPOCK1 was higher in PC-3 M cells. b A Matrigel invasion assay was performed to study the invasive abilities of PC-3 and PC-3 M cells. Quantitative results of counting invaded cells in a 200× field. c SPOCK1 expression was assessed by a Western blot analysis in PC-3 and PC-3 M cells after treatment with various concentrations of API for 24 h. d-f Influences of SPOCK1 on EMT-related marker expressions ( d ) and invasive abilities of PC-3 M ( e ) and PC-3 cells ( f ) in response to API

Article Snippet: Fig. 7 Clinical significance of SPOCK1 in human prostate cancer (PCa). a Left panel: Representative immunohistochemical (IHC) staining for SPOCK1 in a prostate cancer tissue microarray (Biomax.US, PR1921a).

Techniques: Marker, Inhibition, Western Blot, Invasion Assay, Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Targeting the SPOCK1-snail/slug axis-mediated epithelial-to-mesenchymal transition by apigenin contributes to repression of prostate cancer metastasis

doi: 10.1186/s13046-019-1247-3

Figure Lengend Snippet: Targeting SPOCK1 suppresses prostate cancer growth and metastasis by apigenin (API) in an orthotopic mouse model. a Timeline of the in vivo study design for investigating the effects of SPOCK1 expression on tumor progression and the antitumor activity of API. Male NSG mice were orthotopically injected with luciferase-tagged and SPOCK1-depleted (shSPOCK1) PC-3 M cells or SPOCK1-overexpressing PC-3 cells. After 7 days, mice were treated with API (3 mg/kg, IP) or the vehicle for 6 days/week. Whole body bioluminescence imaging was conducted at different time points after cell-injection in mice. b All mice were sacrificed and dissected at 4 weeks after API treatment, and the luciferase activity was detected every week with an IVIS imaging system (left panel). Quantitative analysis of the Xenogen imaging signal intensity (photons/s) every week (right panel). c Tumors were dissected and photographed after 5 weeks (left panel), and the average tumor weight in each group is given (right panel). Data are presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the control group. # p < 0.05, ## p < 0.01, ### p < 0.001 compared to the API-treated group. d-g Representative ex vivo bioluminescence imaging of metastatic sites at the end of this spontaneous metastasis assay. Lungs ( d ), pancreas ( e ), liver ( f ), and bone ( g ) are major organs for metastasis, and signal intensities of metastatic organs were imaged with bioluminescence at the end of the study, with the mean signal for each group indicated. Data are presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. h Kaplan-Meier curves and log-rank test of overall survival analysis for indicated tumor-bearing mice treated with API (3 mg/kg, IP) or the vehicle

Article Snippet: Fig. 7 Clinical significance of SPOCK1 in human prostate cancer (PCa). a Left panel: Representative immunohistochemical (IHC) staining for SPOCK1 in a prostate cancer tissue microarray (Biomax.US, PR1921a).

Techniques: In Vivo, Expressing, Activity Assay, Injection, Luciferase, Imaging, Control, Ex Vivo

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Targeting the SPOCK1-snail/slug axis-mediated epithelial-to-mesenchymal transition by apigenin contributes to repression of prostate cancer metastasis

doi: 10.1186/s13046-019-1247-3

Figure Lengend Snippet: Modulation of SPOCK1 by apigenin (API) interrupted epithelial-to-mesenchymal transition (EMT) activation in vivo. Representative examples of tumors formed in mice injected with the indicated cells . a Histological and IHC analysis of sections from tumor samples for SPOCK1. b, c Western blot analysis of expressions of SPOCK1 and a series of EMT-related proteins in tumor tissues of the orthotopic prostate cancer model

Article Snippet: Fig. 7 Clinical significance of SPOCK1 in human prostate cancer (PCa). a Left panel: Representative immunohistochemical (IHC) staining for SPOCK1 in a prostate cancer tissue microarray (Biomax.US, PR1921a).

Techniques: Activation Assay, In Vivo, Injection, Western Blot

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Targeting the SPOCK1-snail/slug axis-mediated epithelial-to-mesenchymal transition by apigenin contributes to repression of prostate cancer metastasis

doi: 10.1186/s13046-019-1247-3

Figure Lengend Snippet: Clinical significance of SPOCK1 in human prostate cancer (PCa). a Left panel: Representative immunohistochemical (IHC) staining for SPOCK1 in a prostate cancer tissue microarray (Biomax.US, PR1921a). Right panel: Comparison of IHC staining of SPOCK1 in normal prostate tissues and prostate cancer tissues using IHC scores. SPOCK1 expression was significantly higher in PCa tissues than in normal prostate tissues. Black boxes indicate the enlarged area. b Expression of SPOCK1 was assessed in prostate adenocarcinoma patients from GEO database (GSE40272). c SPOCK1 expression was significantly higher in PCa tissues compared to paired normal prostatic tissues from TCGA database. d Kaplan-Meier analysis of SPOCK1 gene expression in the GSE40272 human prostate cancer cohort ( n = 89)

Article Snippet: Fig. 7 Clinical significance of SPOCK1 in human prostate cancer (PCa). a Left panel: Representative immunohistochemical (IHC) staining for SPOCK1 in a prostate cancer tissue microarray (Biomax.US, PR1921a).

Techniques: Immunohistochemical staining, Immunohistochemistry, Microarray, Comparison, Expressing, Gene Expression

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Targeting the SPOCK1-snail/slug axis-mediated epithelial-to-mesenchymal transition by apigenin contributes to repression of prostate cancer metastasis

doi: 10.1186/s13046-019-1247-3

Figure Lengend Snippet: Schematic illustration of the molecular mechanism underlying the ability of apigenin (API) to suppress prostate cancer metastasis. The metastasis-suppressive effect of API on prostate cancer cells was attributed to attenuation of SPOCK1-mediated Snail/Slug expression which resulted in ultimate restraint of EMT progression and subsequent suppression of prostate cancer metastasis

Article Snippet: Fig. 7 Clinical significance of SPOCK1 in human prostate cancer (PCa). a Left panel: Representative immunohistochemical (IHC) staining for SPOCK1 in a prostate cancer tissue microarray (Biomax.US, PR1921a).

Techniques: Expressing